Publications by Costantino Grana

High-resolution histological imaging provides essential detail for quantitative brain modeling, yet acquiring whole-brain data at micrometer scale remains technically and economically challenging. This work introduces Brain-SR, a diffusion-based super-resolution framework designed to reconstruct high-resolution cortical sections from low-resolution BigBrain data. Building upon the InvSR paradigm, our method performs resolution enhancement in the latent space of a pretrained variational autoencoder, guided by a task-specific noise-predictor network. A key contribution is a frequency-domain supervision term that compares the magnitude spectra of predicted and target patches, enforcing spectral consistency while remaining robust to local misalignments. Quantitative evaluations demonstrate that Brain-SR achieves substantial improvements in LPIPS (-27%) and FID (-58%) compared to baseline diffusion Super-Resolution, while spectral analysis confirms accurate recovery of the frequency distribution. The resulting reconstructions preserve neuronal structures consistent with high-resolution references, offering a practical step toward large-scale, morphologically faithful brain histology reconstruction. The code is publicly available to support reproducibility: https://github.com/AImageLab-zip/Brain-SR.

Barcodes are a critical technology in industrial automation, logistics, and retail, enabling fast and reliable data capture. While deep learning has significantly improved barcode localization accuracy, most modern architectures remain too computationally demanding for real-time deployment on embedded systems without dedicated hardware acceleration. In this work, we present BaFaLo (Barcode Fast Localizer), an ultra-lightweight segmentation-based neural network for barcode localization. Our model is specifically optimized for real-time performance on low-power CPUs while maintaining high localization accuracy for both 1D and 2D barcodes. It features a two-branch architecture—comprising a local feature extractor and a global context module—and is tailored for low-resolution inputs to improve inference speed further. We benchmark BaFaLo against several lightweight architectures for object detection or segmentation, including YOLO Nano, Fast-SCNN, BiSeNet V2, and ContextNet, using the BarBeR dataset. BaFaLo achieves the fastest inference time among all deep-learning models tested, operating at 57.62ms per frame on a single CPU core of a Raspberry Pi 3B+. Despite its compact design, it achieves a decoding rate nearly equivalent to YOLO Nano for 1D barcodes and only 3.5 percentage points lower for 2D barcodes while being approximately nine times faster.

CNNs and Transformer-based architectures are recently dominating the field of 3D medical segmentation. While CNNs face limitations in the local receptive field, Transformers require significant memory and data, making them less suitable for analyzing large 3D medical volumes. Consequently, fully convolutional network models like U-Net are still leading the 3D segmentation scenario. Although efforts have been made to reduce the Transformers computational complexity, such optimized models still struggle with content-based reasoning. This paper examines Mamba, a Recurrent Neural Network (RNN) based on State Space Models (SSMs), which achieves linear complexity and has outperformed Transformers in long-sequence tasks. Specifically, we assess Mamba’s performance in 3D medical segmentation using three widely recognized and commonly employed datasets and propose architectural enhancements to improve its segmentation effectiveness by mitigating the primary shortcomings of existing Mamba-based solutions.

This paper provides a detailed description of how to install, set up, and use "BarBeR" (Barcode Benchmark Repository) to reproduce the results presented in the ICPR 2024 paper "BarBeR: A Barcode Benchmarking Repository". The paper details the tests available in the repository and how the configuration parameters affect and influence experimental results.

Since their invention in 1949, barcodes have remained the preferred method for automatic data capture, playing a crucial role in supply chain management. To detect a barcode in an image, multiple algorithms have been proposed in the literature, with a significant increase of interest in the topic since the rise of deep learning. However, research in the field suffers from many limitations, including the scarcity of public datasets and code implementations, which hampers the reproducibility and reliability of published results. For this reason, we developed "BarBeR" (Barcode Benchmark Repository), a benchmark designed for testing and comparing barcode detection algorithms. This benchmark includes the code implementation of various detection algorithms for barcodes, along with a suite of useful metrics. It offers a range of test setups and can be expanded to include any localization algorithm. In addition, we provide a large, annotated dataset of 8748 barcode images, combining multiple public barcode datasets with standardized annotation formats for both detection and segmentation tasks. Finally, we share the results obtained from running the benchmark on our dataset, offering valuable insights into the performance of different algorithms.

We introduce Bits2Bites, the first publicly available dataset for occlusal classification from intra-oral scans, comprising 200 paired upper and lower dental arches annotated across multiple clinically relevant dimensions (sagittal, vertical, transverse, and midline relationships). Leveraging this resource, we propose a multi-task learning benchmark that jointly predicts five occlusal traits from raw 3D point clouds using state-of-the-art point-based neural architectures. Our approach includes extensive ablation studies assessing the benefits of multi-task learning against single-task baselines, as well as the impact of automatically-predicted anatomical landmarks as input features. Results demonstrate the feasibility of directly inferring comprehensive occlusion information from unstructured 3D data, achieving promising performance across all tasks. Our entire dataset, code, and pretrained models are publicly released to foster further research in automated orthodontic diagnosis.

Testicular ultrasound imaging is vital for assessing male infertility, with testicular inhomogeneity serving as a key biomarker. However, subjective interpretation and the scarcity of publicly available datasets pose challenges to automated classification. In this study, we explore supervised and unsupervised pretraining strategies using a ResNet-based architecture, supplemented by diffusion-based generative models to synthesize realistic ultrasound images. Our results demonstrate that pretraining significantly enhances classification performance compared to training from scratch, and synthetic data can effectively substitute real images in the pretraining process, alleviating data-sharing constraints. These methods offer promising advancements toward robust, clinically valuable automated analysis of male infertility. The source code is publicly available at https://github.com/AImageLab-zip/TesticulUS/.

Brain tumor segmentation is a crucial task in medical imaging that involves the integrated modeling of four distinct imaging modalities to identify tumor regions accurately. Unfortunately, in real-life scenarios, the full availability of such four modalities is often violated due to scanning cost, time, and patient condition. Consequently, several deep learning models have been developed to address the challenge of brain tumor segmentation under conditions of missing imaging modalities. However, the majority of these models have been evaluated using the 2018 version of the BraTS dataset, which comprises only $285$ volumes. In this study, we reproduce and extensively analyze the most relevant models using BraTS2023, which includes 1,250 volumes, thereby providing a more comprehensive and reliable comparison of their performance. Furthermore, we propose and evaluate the adoption of Mamba as an alternative fusion mechanism for brain tumor segmentation in the presence of missing modalities. Experimental results demonstrate that transformer-based architectures achieve leading performance on BraTS2023, outperforming purely convolutional models that were instead superior in BraTS2018. Meanwhile, the proposed Mamba-based architecture exhibits promising performance in comparison to state-of-the-art models, competing and even outperforming transformers. The source code of the proposed approach is publicly released alongside the benchmark developed for the evaluation: https://github.com/AImageLab-zip/IM-Fuse.

Convolutional Neural Networks (CNNs) have been broadly employed in dermoscopic image analysis, mainly due to the large amount of data gathered by the International Skin Imaging Collaboration (ISIC). But where do neural networks look? Several authors have claimed that the ISIC dataset is affected by strong biases, i.e. spurious correlations between samples that machine learning models unfairly exploit while discarding the useful patterns they are expected to learn. These strong claims have been supported by showing that deep learning models maintain excellent performance even when "no information about the lesion remains" in the debased input images. With this paper, we explore the interpretability of CNNs in dermoscopic image analysis by analyzing which characteristics are considered by autonomous classification algorithms. Starting from a standard setting, experiments presented in this paper gradually conceal well-known crucial dermoscopic features and thoroughly investigate how CNNs performance subsequently evolves. Experimental results carried out on two well-known CNNs, EfficientNet-B3, and ResNet-152, demonstrate that neural networks autonomously learn to extract features that are notoriously important for melanoma detection. Even when some of such features are removed, the others are still enough to achieve satisfactory classification performance. Obtained results demonstrate that literature claims on biases are not supported by carried-out experiments. Finally, to demonstrate the generalization capabilities of state-of-the-art CNN models for skin lesion classification, a large private dataset has been employed as an additional test set.

The segmentation of the Inferior Alveolar Canal (IAC) plays a central role in maxillofacial surgery, drawing significant attention in the current research. Because of their outstanding results, deep learning methods are widely adopted in the segmentation of 3D medical volumes, including the IAC in Cone Beam Computed Tomography (CBCT) data. One of the main challenges when segmenting large volumes, including those obtained through CBCT scans, arises from the use of patch-based techniques, mandatory to fit memory constraints. Such training approaches compromise neural network performance due to a reduction in the global contextual information. Performance degradation is prominently evident when the target objects are small with respect to the background, as it happens with the inferior alveolar nerve that develops across the mandible, but involves only a few voxels of the entire scan. In order to target this issue and push state-of-the-art performance in the segmentation of the IAC, we propose an innovative approach that exploits spatial information of extracted patches and integrates it into a Transformer architecture. By incorporating prior knowledge about patch location, our model improves state of the art by ~2 points on the Dice score when integrated with the standard U-Net architecture. The source code of our proposal is publicly released.